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Ido1 Antibody

IDO antibody

Product Specifications

Product Name Alternative

Rabbit anti-Ido antibody, rabbit anti-Ido1 antibody, Indoleamine 2,3-dioxygenase 1, Ido-1, Ido1, Ido 1, Indoleamine-pyrrole 2,3-dioxygenase

Reactivity

Mouse

Immunogen

IDO affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of mouse IDO.

Target

Ido1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Concentration

1 mg/ml

Dilution

ELISA: 1: 10,000, WB: 1ug/ml

Purity

Anti-IDO was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with mouse based on 100% sequence homology. Cross-reactivity with IDO from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-IDO antibody is useful for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~41kDa corresponding to the appropriate cell lysate or extract.

Tested Applications

ELISA, WB

Host or Source

Rabbit

Preservative

Preservative: 50% (v/v) Glycerol with 1 mg/ml Bovine Serum Albumin (rAlbumin) ; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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