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Edc3 Antibody

EDC3 pS161 antibody

Product Specifications

Product Name Alternative

Rabbit anti-EDC3 pS161 Antibody, Enhancer of mRNA-decapping protein 3, YjeF domain-containing protein 1, Edc-3, Edc 3, Yjdc

UniProt

Q8K2D3

Reactivity

Mouse

Immunogen

EDC3 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide surrounding the pS161 site of mouse EDC3.

Target

Edc3

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.0 mg/ml

Dilution

ELISA: 1: 10,000, WB: 0.5ug/ml

Purity

Anti-EDC3 pS161 is specific for phospho S161 mouse EDC3. It was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with rabbit, pig, monkey, bovine, rat, mouse, and bat based on 100% sequence homology. Cross-reactivity with EDC3 pS161 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-EDC3pS161 antibody is useful for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~55kDa corresponding to the appropriate cell lysate or extract.

Tested Applications

ELISA, WB

Host or Source

Rabbit

Preservative

Preservative: 0.05% (w/v) Sodium Azide. Stabilizer: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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