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HDAC5 Antibody

HDAC-5 antibody

Product Specifications

Product Name Alternative

Rabbit anti-HDAC-5 antibody, HDAC5, HDAC 5, HD 5 antibody, histone deacetylase 5 antibody, Histone deacetylase-5, HD5 antibody, NY-CO-9

UniProt

Q9UQL6

Reactivity

Human, Mouse

Immunogen

HDAC5 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding phosphoserine 661 of human HDAC5.

Target

HDAC5

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Concentration

1.12 mg/mL

Dilution

ELISA: 1:20,000 - 1:60,000, IHC: 1:100-1:500, IF: 1:100-1:500, WB: 0.2 μg/ml

Purity

Anti-HDAC5 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards HDAC5. A BLAST analysis was used to suggest cross-reactivity with Human based on 100% sequence homology. Cross-reactivity with HDAC5 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-HDAC5 antibody has been tested in ELISA, dot blot, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~124 kDa corresponding to the appropriate cell lysate or extract.

Tested Applications

DOT, ELISA, IF, IHC, WB

NCBI Accession Number

NP_005465.2

Host or Source

Rabbit

Preservative

Preservative: None. Stabilizer: 50% (v/v) Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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