CANX Antibody
Calnexin antibody
Product Specifications
Product Name Alternative
Rabbit anti-calnexin antibody, CANX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90
UniProt
P27824
Reactivity
Gallus, Human, Mouse, Primate, Rat
Immunogen
Calnexin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human Calnexin.
Target
CANX
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Concentration
0.34 mg/ml
Dilution
ELISA: 1:20,000 - 1:60,000, IHC: 1:100-1:500, IF: 1:100-1:500, WB: 0.2 μg/ml
Purity
Anti-Calnexin was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards calnexin. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat, Primate and Chicken based on 100% sequence homology. Cross-reactivity with calnexin from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Calnexin antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~67.7 kDa corresponding to the appropriate cell lysate or extract.
Tested Applications
ELISA, IF, IHC, WB
NCBI Accession Number
NP_001019820.1
Host or Source
Rabbit
Preservative
Preservative: None. Stabilizer: 50% (v/v) Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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