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APP Antibody

Beta Amyloid pyro E3 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Beta Amyloid pyro E3 Antibody, ß-amyloid pyro E3, Alzheimer disease amyloid protein, Beta amyloid, A-beta, ABPP, APPI

UniProt

P05067

Reactivity

Human

Immunogen

Beta Amyloid [Pyro Glu3] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-terminus 3-pyro E start point of human beta Amyloid.

Target

APP

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Concentration

1.0 mg/mL

Dilution

ELISA: 1:20,000 - 1:60,000, IHC: 1:100-1:500, IF: 1:100-1:500, WB: 1 μg/ml

Purity

Anti-Beta Amyloid pyro Glu3 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody contains no reactivity towards the 1-42 ABeta peptide. A BLAST analysis was used to suggest cross-reactivity with Human, Primate, Bovine and Sheep based on 100% sequence homology. It is not reactive with Rodentia. Cross-reactivity with beta Amyloid pyro Glu3 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Beta Amyloid pyro Glu3 has been tested in dot blot, ELISA, Western Blot, and Immunostaining. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~86.9kDa corresponding to the appropriate cell lysate or extract.

Tested Applications

DOT, ELISA, IF, IHC, Multiplex Assay, WB

NCBI Accession Number

NP_000475.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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