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APP Antibody

Beta Amyloid pyro E11 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Beta Amyloid pyro E11 Antibody, ß-amyloid pyro E11, Alzheimer disease amyloid protein, Beta amyloid, A-beta, ABPP, APPI

UniProt

P05067

Reactivity

Human, Primate

Immunogen

Beta Amyloid [Pyro Glu11] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-terminus 11-pyro E start point of human beta Amyloid.

Target

APP

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Concentration

0.75 mg/mL

Dilution

ELISA: 1:20,000 - 1:60,000, IHC: 1:100-1:500, IF: 1:100-1:500, WB: 0.2 μg/ml

Purity

Anti-Beta Amyloid pyro Glu11 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific to the N-terminus 11-pyro E start point. A BLAST analysis was used to suggest cross-reactivity with Human and Primate based on 100% sequence homology. Cross-reactivity with beta Amyloid pyro Glu11 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Beta Amyloid pyro Glu11 antibody has been tested by western blot and is useful for ELISA, Western Blot and Immunostaining. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~86.9kDa corresponding to the appropriate cell lysate or extract.

Tested Applications

ELISA, IF, IHC, WB

NCBI Accession Number

NP_000475.1

Host or Source

Rabbit

Preservative

Preservative: None. Stabilizer: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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