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Arhgap22 Antibody

ARHGAP22 (phospho-S22) antibody

Product Specifications

Product Name Alternative

Rabbit anti-ARHGAP22 pS22 Antibody, Rho-type GTPase-activating protein 22, RHOGAP2, Rho GTPase activating protein 22, rho GTPase-activating protein 22, ARHGAP 22, ARHGAP-22

UniProt

Q8BL80

Reactivity

Human, Mouse, Rat

Immunogen

ARHGAP22 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide corresponding to the region surrounding mouse pS22 region of ARHGAP22.

Target

Arhgap22

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Concentration

1.07 mg/ml

Dilution

ELISA: 1:20,000 - 1:60,000, IHC: 1:100-1:500, IF: 1:100-1:500, WB: 1 μg/ml

Purity

Anti-ARHGAP22 pS22 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for phosphorylated ARHGAP22 at Serine 22. It also recognizes the S397->A mutation but not the S22->A mutation. A BLAST analysis was used to suggest cross-reactivity with Mouse, Rat and Human based on 100% sequence homology. Cross-reactivity with ARHGAP22 pS22 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-ARHGAP22 pS22 antibody is tested in Western Blot and ELISA useful for Immunostaining. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77.8 kDa corresponding to the appropriate cell lysate or extract.

Tested Applications

ELISA, IF, IHC, WB

NCBI Accession Number

NP_722495

Host or Source

Rabbit

Preservative

Preservative: None. Stabilizer: 50% (v/v) Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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