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STAT3 Antibody

STAT3 antibody

Product Specifications

Product Name Alternative

Rabbit anti-STAT3 antibody, STAT-3, STAT 3, Signal transducer and activator of transcription 3, Acute-phase response factor, APRF

UniProt

P40763

Reactivity

Human

Immunogen

STAT3 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic monomethylated or asymmetric dimethylated peptides surrounding Arginine 31 of human STAT.

Target

STAT3

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Concentration

1.0 mg/ml

Dilution

ELISA: 1:5000, WB: 1:500

Purity

Anti-STAT3 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with all STAT3 modifications. Cross-reactivity is seen against STAT1 and STAT4. BLAST analysis was used to suggest cross-reactivity with Human, mouse, and rat. Cross-reactivity with STAT3 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-STAT3 antibody has been tested by Dot Blot and is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~88 kDa corresponding to STAT3 protein by Western Blotting in the appropriate cell lysate or extract.

Tested Applications

DOT, ELISA, WB

Host or Source

Rabbit

Preservative

Preservative: 0.05% (w/v) Sodium Azide. Stabilizer: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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