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STAT3 Antibody

STAT3 R31-Me2a antibody

Product Specifications

Product Name Alternative

Rabbit anti-STAT3 Asym-dimethyl Arg31 antibody, rabbit anti-STAT3 R31me2a antibody, Signal transducer and activator of transcription 3, Acute-phase response factor, APRF

UniProt

P40763

Reactivity

Human

Immunogen

STAT3 [Asym-dimethyl Arg31] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Arginine 31 of human STAT.

Target

STAT3

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Concentration

0.83 mg/ml

Dilution

ELISA: 1:5000, WB: 0.1-0.05 μg/ml

Purity

Anti-STAT3 R31-Me2a was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human STAT3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and rat. Cross-reactivity with STAT3 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-STAT3 [Asym-dimethyl Arg31] antibody has been tested in Dot Blot and is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~88 kDa corresponding to STAT3 protein by Western Blotting in the appropriate cell lysate or extract.

Tested Applications

DOT, ELISA, WB

Host or Source

Rabbit

Preservative

Preservative: 0.05% (w/v) Sodium Azide. Stabilizer: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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