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Ctnnb1 Antibody

Beta Catenin antibody

Product Specifications

Product Name Alternative

Rabbit anti-beta Catenin Antibody, rabbit anti-Catenin beta antibody, catenin beta-1, ß-catenin-1, ß-catenin, CTNNB1, CTNNB, beta-catenin antibody

UniProt

F1QGH7

Reactivity

Human, Zebrafish

Immunogen

Beta catenin antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to catenin beta-1 C-terminus.

Target

Ctnnb1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Concentration

1.1 mg/mL

Dilution

ELISA: 1:75,000 - 1:125,000, IHC: 5 - 10 ug/mL, WB: 1:500

Purity

Beta catenin antibody is directed against catenin beta-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with catenin beta-1 protein from human, mouse, rat, bovine, Xenopus laevis, and Danio rerio at 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Beta catenin antibody has been tested for use in ELISA, western blotting, immunohistochemistry, and ISH. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 85.5 kDa in size corresponding to catenin beta-1 protein by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_001091679.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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