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SAE1 Antibody

SAE1 antibody

Product Specifications

Product Name Alternative

Rabbit anti-SAE1 antibody, rabbit anti-SUMO activating enzyme subunit 1 antibody, Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, SUA1, SAE-1

UniProt

Q9UBE0

Reactivity

Human

Immunogen

This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human SUMO Activating Enzyme E1 protein.

Target

SAE1

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

0.41 mg/mL

Dilution

ELISA: 1:5,000 - 1:20,000, WB: 2μg/mL

Purity

This purified antibody is directed against human SUMO Activating Enzyme E1 protein. The product was purified from monospecific antiserum by affinity chromatography. This antibody is specific for human SAE1 protein. A BLAST analysis using the sequence of the immunizing peptide was used to suggest that this antibody would react with SUMO Activating Enzyme E1 protein from human (100%), bovine, dog, chimpanzee (96%), mouse (93%), and rat (92%) based on a high degree of sequence homology. Cross reactivity against this protein from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~37 kDa in size corresponding to SAE1 protein by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, WB

NCBI Accession Number

42559897

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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