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BrdU Antibody

BrdU antibody

Product Specifications

Product Name Alternative

Rabbit anti-BrdU Antibody, bromodeoxyuridine, 5-bromo-2'-deoxyuridine

Reactivity

Other

Immunogen

Anti-BrdU affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with BromodeoxyUridine-KLH.

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.23 mg/mL

Dilution

ELISA: 1:2000 - 1:10,000, FC: 1:50-1:100, IHC: 1:100-1:500, IF: 1:500

Purity

Anti-BrdU Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Form

Liquid (sterile filtered)

Storage Conditions

Store BrdU Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-BrdU Antibody has been tested by ELISA, slot blot, IHC, and is suitable for immunofluorescence microscopy and flow cytometry. Specific conditions for reactivity should be optimized by the end user. Expect to detect incorporated BrdU thymidine analog from replicated cells

Tested Applications

DOT, ELISA, FC, IF, IHC

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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