BrdU Antibody
BrdU antibody
Product Specifications
Product Name Alternative
Rabbit anti-BrdU Antibody, bromodeoxyuridine, 5-bromo-2'-deoxyuridine
Reactivity
Other
Immunogen
Anti-BrdU affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with BromodeoxyUridine-KLH.
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.23 mg/mL
Dilution
ELISA: 1:2000 - 1:10,000, FC: 1:50-1:100, IHC: 1:100-1:500, IF: 1:500
Purity
Anti-BrdU Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Form
Liquid (sterile filtered)
Storage Conditions
Store BrdU Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-BrdU Antibody has been tested by ELISA, slot blot, IHC, and is suitable for immunofluorescence microscopy and flow cytometry. Specific conditions for reactivity should be optimized by the end user. Expect to detect incorporated BrdU thymidine analog from replicated cells
Tested Applications
DOT, ELISA, FC, IF, IHC
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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