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THRA Antibody

THRA antibody

Product Specifications

Product Name Alternative

Rabbit anti-THRA antibody, rabbit anti-Thyroid hormone receptor alpha antibody, Nuclear receptor subfamily 1 group A member 1, c-erbA-alpha, c-erbA-1, V-erbA-related protein 7, EAR7, ERBA1, NR1A1, THRA1, THRA2

UniProt

P10827

Reactivity

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human THRA isoform 1 protein.

Target

THRA

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

0.89 mg/mL

Dilution

ELISA: 1:300,000, IHC: 1:200, IF: 1:200, IP: 1:100, WB: 1:500 - 1:2,000

Purity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human THRA protein and recognizes THRA isoform 1. A BLAST analysis was used to suggest cross-reactivity with THRA from mouse, human and rat based on a 100% homology with the immunizing sequence. Cross-reactivity with THRA from other sources and isoforms may occur but has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

This affinity purified antibody has been tested for use in ELISA and western blotting.

Tested Applications

ELISA, IF, IHC, IP, WB

NCBI Accession Number

40806160

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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