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NOXO1 Antibody

NOXO1 antibody

Product Specifications

Product Name Alternative

Rabbit anti-NOXO1 antibody, NOXO-1, NOXO 1, NADPH oxidase organizer 1 antibody, NADPH oxidase regulatory protein antibody, Nox organizer 1 antibody, Nox organizing protein 1 antibody, Regulatory protein P41NOX antibody, SH3 and PX domain-containing protein 5, P41NOX, SH3PXD5

UniProt

Q8NFA2

Reactivity

Human

Immunogen

NOXO1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 238-252 of human NOXO1 protein.

Target

NOXO1

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.0mg/ml

Dilution

ELISA: 1:20,000 - 1:100,000, IHC: 5-10 μg/ml, WB: 1:1,000 - 1:10,000

Purity

Anti-NOXO-1 antibody is directed against human NOXO1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with NOXO1 protein from human and chimpanzee (100% homology) . Expect reactivity with alpha, delta and gamma isoforms of NOXO1. Also expect partial reactivity against NOXO1 homologues from dog and guinea pig (87%), as well as rat (75%) and mouse (68%) . Reactivity against homologues from other sources is not known.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-NOXO1 antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to NOXO1 protein by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

16198473

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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