What is the catalog number for AURKB Antibody?

The catalog number for AURKB Antibody is orb345466.

What sizes are available for AURKB Antibody?

AURKB Antibody is available in the following size(s): 25 µL.

Is AURKB Antibody in stock?

AURKB Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

AURKB Antibody

Aurora B (phospho-pT232) antibody

Product Specifications

Product Name Alternative

Rabbit anti-Aurora B pT232 Antibody, AIK2 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, AURKB antibody, Aurora 1 antibody, Aurora and Ipl1 like midbody associated protein 1 antibody, Aurora Kinase B, IPL1-related kinase 2, Aurora-related kinase 2, STK-1, Aurora-B, Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2

UniProt

Q96GD4

Reactivity

Human, Monkey

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding T232 of Human Aurora Kinase B protein.

Target

AURKB

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Concentration

1.0 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, WB: 1:250 - 1:2,000

Purity

Aurora B Phospho pT232 Antibody is affinity purified antibody is directed against the phosphorylated form of human Aurora Kinase B at the pT232 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Aurora Kinase B pT232 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA. No reaction is expected against Aurora Kinase A. However, 100% sequence homology as indicated by BLAST analysis is on record for this protein from human, mouse, rat, cow, pig, dog and chimpanzee. Cross reactivity with Aurora Kinase B from other sources is not known.

Form

Liquid (sterile filtered)

Storage Conditions

Store Phospho Specific Antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Aurora B pT232 Antibody is affinity-purified antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract. HeLa cell lysate can be used as a positive control.