What is the catalog number for AURKB Antibody?

The catalog number for AURKB Antibody is orb345465.

What sizes are available for AURKB Antibody?

AURKB Antibody is available in the following size(s): 100 µg.

Is AURKB Antibody in stock?

AURKB Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

AURKB Antibody

Aurora B (phospho-pT232) antibody

Product Specifications

Product Name Alternative

Rabbit anti-Aurora B pT232 Antibody, Phospho Aurora B, AIK2 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, AURKB antibody, Aurora 1 antibody, Aurora and Ipl1 like midbody associated protein 1 antibody

UniProt

Q96GD4

Reactivity

Human, Monkey

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding T232 of Human Aurora Kinase B protein.

Target

AURKB

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Concentration

0.87 mg/mL

Dilution

ELISA: 1:10,000 - 1:30,000, WB: 1:250 - 1:2,000

Purity

Anti-Phospho Aurora B pT232 affinity purified antibody is directed against the phosphorylated form of human Aurora Kinase B at the pT232 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Aurora Kinase B pT232 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA. No reaction is expected against Aurora Kinase A. However, 100% sequence homology as indicated by BLAST analysis is on record for this protein from human, mouse, rat, cow, pig, dog and chimpanzee. Cross reactivity with Aurora Kinase B from other sources is not known.

Form

Liquid (sterile filtered)

Storage Conditions

Store Phospho Specific Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Phospho pT232 Aurora B antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. See below for specific protocol. Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract. HeLa cell lysate can be used as a positive control.