AURKB Antibody

Rabbit anti-Aurora B Antibody, AIK2 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, AURKB antibody, Aurora 1 antibody, Aurora and Ipl1 like midbody associated protein 1 antibody

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 225-250 of Human Aurora Kinase B protein.

Aurora B antibody is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human Aurora Kinase B protein. No reaction is expected against Aurora Kinase A. However, 100% sequence homology as indicated by BLAST analysis is on record for this protein from human, mouse, rat, cow, pig, dog and chimpanzee. Cross reactivity with Aurora Kinase B from other sources is not known.

Store Anti-Aurora B at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Aurora B is affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract. Human brain tissue lysate or HeLa cell lysate can be used as a positive control.