PAK1 Antibody

Rabbit anti-PAK pT423 antibody, rabbit anti-PAK1/2/3 pT423 antibody, PAK1, PAK-1, PAK 1, Alpha PAK, p21-activated kinase 1, p65-PAK, PAK2, PAK-2, PAK 2, PAK65, S6/H4 kinase, p21-activated kinase 2, p58, Gamma PAK, PAK3, PAK-3, PAK 3, Beta PAK, CDC42/RAC effector kinase PAK-B, p21-activated kinase 3

This affinity purified antibody is directed against human PAK (p21-GTPase Activated Protein Kinase) . The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pT423 of Human PAK 1/2/3. Reactivity with non-phosphorylated PAK 1/2/3 is less than 1% by ELISA. Cross reactivity with PAK 1/2/3 from other species has not been determined.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This phospho specific polyclonal antibody was tested by Dot blot and ELISA and was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional in immunohistochemistry, immunoblotting, and immunoprecipitation. Lysates from Jurkat cells or PAK transfected cells may be used as a control. This product has been assayed against 0.1 μg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:25,000 is suggested for this product. Less than 0.2% cross-reactivity was detected against the non-phosphorylated form of the immunizing peptide. Researchers should determine optimal titers for other applications.