Casp2 Antibody

Rabbit anti-Caspase-2 Antibody, Apoptosis related cysteine peptidase antibody, CASP-2, CASP 2 antibody, Casp 2L Pro antibody, CASP2 antibody, Caspase 2 apoptosis related cysteine protease antibody, Caspase2 antibody, Caspase-2, Developmentally down regulated 2 antibody, ICH 1 antibody, ICH-1, Caspase-2 subunit p18, Caspase-2 subunit p13, Caspase-2 subunit p12

A synthetic peptide corresponding to the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.

This affinity purified antibody is directed against rat and mouse caspase-2. The antibody detects long and short forms of the protein. Antiserum was purified against the immunizing peptide. This antibody reacts with mouse and rat caspase-2, but not caspase-2 from human sources. Cross reactivity with caspase-2 from other sources has not been determined. Rat and mouse caspase-2 show 94% homology. There is no significant homology to the human form.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified polyclonal antibody was tested by ELISA and immunoblotting and was found to be reactive with 48 kDa mouse and rat caspase-2. A control and rat caspase-2 transfected human 293 cell lysate was used to show by immunoblotting that this antibody detects rat caspase-2. No reaction was observed against human caspase-2. Immunoblot of mouse neuronal cell lysates from wild-type and caspase-2 (-/-) mice (not shown) was used to show that this antibody cross reacts with mouse caspase-2. A 1:750 dilution is recommended for immunoblot. This product was assayed against 1.0 μg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product.