COL6A1-COL6A3 Antibody
Collagen Type VI antibody
Product Specifications
Product Name Alternative
Rabbit anti-Collagen Type VI antibody, Collagen alpha-1 (VI) chain, Collagen VI antibody, Human mRNA for collagen VI alpha 1 C terminal globular domain antibody
UniProt
P12109
Reactivity
Bovine, Human
Immunogen
Collagen Type VI from human and bovine placenta
Target
COL6A1-COL6A3
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.19 mg/mL
Dilution
ELISA: 1:5,000 - 1:50,000, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:1,000 - 1:10,000
Purity
Anti-Collagen Type VI has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Collagen Type VI has been tested by dot blot and IHC-P and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis
Tested Applications
DOT, ELISA, IHC, IP, WB
NCBI Accession Number
AAH52575.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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