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TF Antibody

Transferrin antibody

Product Specifications

Product Name Alternative

Rabbit anti-Transferrin antibody, Beta 1 metal binding globulin antibody, PRO1400 antibody, Serotransferrin antibody, Siderophilin antibody, TF antibody

UniProt

P02787

Reactivity

Human

Immunogen

Human Transferrin

Target

TF

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.0 mg/mL

Dilution

ELISA: 1:20,000 - 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigen resins. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.

Form

Liquid (sterile filtered)

Storage Conditions

This product is stable for several weeks at 4° C as an undiluted liquid. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Notes

For research use only.

Applications Notes

Anti-Human transferrin has been tested by ELISA and is assayed against 1.0 μg of Human Transferrin in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified Donkey anti-Rabbit IgG [H&L] MX and TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:43,000 is suggested for this product.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

AAH59367.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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