RNF2 Antibody
RING1B antibody
Product Specifications
Product Name Alternative
Goat anti-RING1B Antibody, RING finger protein BAP1 antibody, DING antibody, RING finger protein 1B, E3 ubiquitin protein ligase RING 2 antibody, Huntingtin interacting protein 2 interacting protein 3 antibody, Anti-RNF2 antibody, HIP2-interacting protein 3, Protein DinG
UniProt
Q99496
Reactivity
Chinese Hamster, Human, Mouse
Immunogen
RING1B Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 180-205 of human RING1B protein.
Target
RNF2
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.1 mg/mL
Dilution
ELISA: 1:5,000 - 1:25,000, IF: 1:300, WB: 1:500 - 1:2,000
Purity
Affinity purified Anti-RING1B antibody is directed against human RING1B protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, mouse, rat, dog, bovine, frog and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with RING1B from zebrafish, as only a single amino acid residue changes within the immunogen sequence (92% positive by BLAST) . Cross reactivity with RING1B homologues from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-RING1B purified antibody has been tested for use in ELISA, immunofluorescence, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size corresponding to RING1B by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IF, Multiplex Assay, WB
NCBI Accession Number
6005747
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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