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RNF2 Antibody

RING1B antibody

Product Specifications

Product Name Alternative

Goat anti-RING1B Antibody, RING finger protein BAP1 antibody, DING antibody, RING finger protein 1B, E3 ubiquitin protein ligase RING 2 antibody, Huntingtin interacting protein 2 interacting protein 3 antibody, Anti-RNF2 antibody, HIP2-interacting protein 3, Protein DinG

UniProt

Q99496

Reactivity

Chinese Hamster, Human, Mouse

Immunogen

RING1B Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 180-205 of human RING1B protein.

Target

RNF2

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.1 mg/mL

Dilution

ELISA: 1:5,000 - 1:25,000, IF: 1:300, WB: 1:500 - 1:2,000

Purity

Affinity purified Anti-RING1B antibody is directed against human RING1B protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, mouse, rat, dog, bovine, frog and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with RING1B from zebrafish, as only a single amino acid residue changes within the immunogen sequence (92% positive by BLAST) . Cross reactivity with RING1B homologues from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-RING1B purified antibody has been tested for use in ELISA, immunofluorescence, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size corresponding to RING1B by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, IF, Multiplex Assay, WB

NCBI Accession Number

6005747

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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