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APOC3 Antibody

APOLIPOPROTEINC-III antibody

Product Specifications

Product Name Alternative

Goat anti-Apolipoprotein C-III antibody, ApoC-III, Apo-CIII antibody, Apolipoprotein C3, Apolipoprotein C III antibody

UniProt

P02656

Reactivity

Human

Immunogen

Produced from apoLipoprotein Type C-III derived from human plasma

Target

APOC3

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Concentration

1.0 mg/mL

Dilution

ELISA: 1:5,000 - 1:10,000, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:500 - 1:5,000

Purity

This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein C-III and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Apolipoprotein antibodies have been tested in immunohistochemistry and used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, and for western blotting for highly sensitive qualitative analysis. When performing immunoblotting assays - do not block with blotto. Blotto formulations tend to contain apoLipoprotein Type C-III and will neutralize antibody activity.

Tested Applications

ELISA, IHC, IP, WB

NCBI Accession Number

CAA25648.1

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Isotype

IgG

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