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APOB Antibody

APOLIPOPROTEIN B antibody

Product Specifications

Product Name Alternative

Goat anti-Apolipoprotein B antibody, Apo B-100, Apo B-48, APOB protein antibody, Apolipoprotein-B 100 antibody, Apolipoprotein B 48 antibody, Apolipoprotein B antibody, FLDB antibody

UniProt

Q7Z7Q0

Reactivity

Human

Immunogen

ApoLipoprotein Type B was isolated from human plasma by density gradient centrifugation followed by HPLC purification.

Target

APOB

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Concentration

1.0 mg/mL

Dilution

ELISA: 1:2,000 - 1:10,000, IHC: 1:50 - 1:500, IP: 1:100, WB: 1:200 - 1:1,000

Purity

This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.

Tested Applications

ELISA, IHC, IP, WB

NCBI Accession Number

AAH51278.1

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Isotype

IgG

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