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GFP Antibody Rhodamine Conjugated

GFP antibody (TRITC)

Product Specifications

Product Name Alternative

Goat anti-GFP Antibody Rhodamine Conjugation, TRITC conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

UniProt

P42212

Reactivity

Other

Immunogen

Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Clonality

Polyclonal

Conjugation

TRITC

Concentration

1.0 mg/mL

Dilution

FLISA: >1:20,000, IF: 1:500 - 1:2,500, WB: >1:10,000

Purity

GFP Rhodamine Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.

Form

Lyophilized

Storage Conditions

Store Anti GFP at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Goat Anti-GFP Rhodamine Conjugated Antibody has been tested by dot blot and western blot and is useful in immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Tested Applications

DOT, FLISA, IF, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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