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Fluorescein Antibody Rhodamine Conjugated

Fluorescein antibody (TRITC)

Product Specifications

Product Name Alternative

Goat Anti-FITC antibody Rhodamine conjugation, TRITC conjugated goat anti-fluorescein antibody, TRITC conjugated goat Anti-FITC antibody, goat anti-fluorescein antibody Rhodamine conjugation

Immunogen

Fluorescein conjugated to Goat IgG

Clonality

Polyclonal

Conjugation

TRITC

Concentration

1.0 mg/mL

Dilution

FLISA: >1:20,000, IF: 1:500 - 1:2,500, WB: >1:10,000

Purity

Fluorescein Antibody Conjugated to Rhodamine was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein Conjugated IgG.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Fluorescein Rhodamine Conjugated Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Tested Applications

FLISA, IF, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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