IL17a Antibody
IL-17A antibody
Product Specifications
Product Name Alternative
Rat anti-IL-17A antibody, rat anti-interleukin17A antibody, Interleukin-17A cytokine, IL-17A, IL-17, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8
UniProt
Q62386
Reactivity
Mouse
Immunogen
This Mouse IL-17A monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse IL-17A protein (produced in E.coli) followed by hybridoma development.
Target
IL17a
Clonality
Monoclonal
Clone
20B4.G10.F5
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
2.94 mg/mL
Dilution
ELISA: 1:10,000, FC: 1:500, WB: 1:1000
Purity
This monoclonal antibody is purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse IL-17A protein. Cross-reactivity with IL-17A from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store IL-17A antibody at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
IL-17 A antibody has been tested for use in western blotting, Flow Cytometry and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23 kDa in size corresponding to the mature mouse IL-17A protein, a non-glycosylated polypeptide chain consisting of 207 amino acids, by western blotting in appropriate cell lysate or extract.
Tested Applications
ELISA, FC, Multiplex Assay, WB
NCBI Accession Number
NP_034682.1
Host or Source
Rat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG2a
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