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IL17a Antibody

IL-17A antibody

Product Specifications

Product Name Alternative

Rat anti-IL-17A antibody, rat anti-interleukin17A antibody, Interleukin-17A cytokine, IL-17A, IL-17, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8

UniProt

Q62386

Reactivity

Mouse

Immunogen

This Mouse IL-17A monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse IL-17A protein (produced in E.coli) followed by hybridoma development.

Target

IL17a

Clonality

Monoclonal

Clone

20B4.G10.F5

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Concentration

2.94 mg/mL

Dilution

ELISA: 1:10,000, FC: 1:500, WB: 1:1000

Purity

This monoclonal antibody is purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse IL-17A protein. Cross-reactivity with IL-17A from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store IL-17A antibody at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

IL-17 A antibody has been tested for use in western blotting, Flow Cytometry and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23 kDa in size corresponding to the mature mouse IL-17A protein, a non-glycosylated polypeptide chain consisting of 207 amino acids, by western blotting in appropriate cell lysate or extract.

Tested Applications

ELISA, FC, Multiplex Assay, WB

NCBI Accession Number

NP_034682.1

Host or Source

Rat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG2a

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