Nestin Rabbit Polyclonal Antibody

Product Specifications

Product Name Alternative

Nestin; NES; Nbla00170

UniProt

P48681

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Nestin, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

Target

Nestin

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology, Cardiovascular Research, Cell Biology, Disease Biomarkers, Neuroscience, Stem Cell & Developmental Biology

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Dilution

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Western blot, 0.1-0.5μg/ml

Form

Lyophilized

Molecular Weight

230 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Tested Applications

IHC, WB

Host or Source

Rabbit

Preservative

Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.