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IL33 Antibody

IL-33 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Interleukin-33 antibody, rabbit anti-IL-33 antibody, Interleukin-1 family member 11, IL-1F11, nuclear factor from high endothelial venules and NF-HEV

UniProt

O95760

Reactivity

Human

Immunogen

This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-33 protein.

Target

IL33

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Concentration

1.0 mg/mL

Dilution

ELISA: 1:20,000 - 1:100,000, WB: 1:500 - 1:2,000

Purity

This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-33 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/μl by the LAL method. By western blot a band approximately 18 kDa in size corresponding to mature human IL-32α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Tested Applications

ELISA, WB

NCBI Accession Number

15559209

Host or Source

Rabbit

Preservative

Preservative: None. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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