IL17A Antibody
IL-17A antibody
Product Specifications
Product Name Alternative
Rabbit anti-Interleukin-17A antibody, rabbit anti-IL-17A antibody, IL-17A, Interleukin-17A, Cytotoxic T-Lymphocyte-associated Antigen 8, CTLA8, Interleukin-17, IL17, IL-17, Interleukin17
UniProt
Q16552
Reactivity
Human
Immunogen
IL-17A Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL17-A protein.
Target
IL17A
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
1.0 mg/mL
Dilution
ELISA: 1:1:000 - 1:5,000, IHC: 1:100, WB: 1:500 - 1:2,000
Purity
Anti-IL-17A Antibody was affinity purified from monospecific antiserum by Protein A Purification. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended. Cross-reactivity with IL-17 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
IL-17-A antibody has been tested for use in IHC and western blotting and is suitable in ELISA. By western blot a band approximately ~17.5 kDa in size corresponding to human IL-17-A protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
AAH662531.1
Host or Source
Rabbit
Preservative
Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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