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IL17A Antibody

IL-17A antibody

Product Specifications

Product Name Alternative

Rabbit anti-Interleukin-17A antibody, rabbit anti-IL-17A antibody, IL-17A, Interleukin-17A, Cytotoxic T-Lymphocyte-associated Antigen 8, CTLA8, Interleukin-17, IL17, IL-17, Interleukin17

UniProt

Q16552

Reactivity

Human

Immunogen

IL-17A Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL17-A protein.

Target

IL17A

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Concentration

1.0 mg/mL

Dilution

ELISA: 1:1:000 - 1:5,000, IHC: 1:100, WB: 1:500 - 1:2,000

Purity

Anti-IL-17A Antibody was affinity purified from monospecific antiserum by Protein A Purification. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended. Cross-reactivity with IL-17 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

IL-17-A antibody has been tested for use in IHC and western blotting and is suitable in ELISA. By western blot a band approximately ~17.5 kDa in size corresponding to human IL-17-A protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

AAH662531.1

Host or Source

Rabbit

Preservative

Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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