BIN1 Antibody
BIN1 antibody
Product Specifications
Product Name Alternative
AMPHL, Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, BIN 1, BIN-1, mouse anti-BIN1 antibody, anti-BIN1 antibody
UniProt
O00499
Reactivity
Human
Immunogen
Anti-BIN1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with 12A exon BIN1 protein followed by hybridoma development.
Target
BIN1
Clonality
Monoclonal
Clone
12A
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5000 - 1:50000, WB: 1:500-1:1500
Purity
Anti-BIN1 purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human BIN1 protein. A BLAST analysis was used to suggest cross-reactivity with BIN1 from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with BIN1 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-BIN1 antibody has been tested for use in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_004296.1
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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