CXCL10 Antibody

This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 83 amino acids of the mature bovine CXCL10 protein.

This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for bovine CXCL10 protein. A BLAST analysis was used to suggest cross-reactivity with CXCL10 from bovine based on 100% homology with the immunizing sequence. Partial reactivity is expected against sheep and goat based on 94-95% homology. Cross-reactivity with CXCL10 from other sources has not been determined.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

This protein-A purified CXCL10 antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 9.3 kDa in size corresponding to bovine CXCL10 protein by western blotting in the appropriate cell lysate or extract.