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MDH1 Antibody (HRP)

Malate Dehydrogenase antibody (Peroxidase)

Product Specifications

Product Name Alternative

Sheep anti-Malate Dehydrogenase HRP conjugated Antibody, peroxidase conjugated sheep anti-Malate Dehydrogenase Antibody, cytoplasmic, Cytosolic malate dehydrogenase

UniProt

P11708

Reactivity

Porcine

Immunogen

Malate Dehydrogenase [Pig Heart]

Target

MDH1

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Metabolism Research

Concentration

1 mg/ml

Dilution

ELISA: 1:1,000 - 1:5,000, IHC: 1:50 - 1:250, IP: 1:50, WB: 1:1,000 - 1:5,000

Purity

Malate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart]. Cross reactivity against Malate Dehydrogenase from other sources may occur but has not been specifically determined.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Malate Dehydrogenase Peroxidase Conjugated has been tested by western blot and is useful in ELISA and immunohistochemistry. Optimal titers should be obtained by researchers.

Tested Applications

ELISA, IHC, IP, WB

NCBI Accession Number

P11708.4

Host or Source

Sheep

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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