MDH1 Antibody (HRP)
Malate Dehydrogenase antibody (Peroxidase)
Product Specifications
Product Name Alternative
Sheep anti-Malate Dehydrogenase HRP conjugated Antibody, peroxidase conjugated sheep anti-Malate Dehydrogenase Antibody, cytoplasmic, Cytosolic malate dehydrogenase
UniProt
P11708
Reactivity
Porcine
Immunogen
Malate Dehydrogenase [Pig Heart]
Target
MDH1
Clonality
Polyclonal
Conjugation
HRP
Field of Research
Metabolism Research
Concentration
1 mg/ml
Dilution
ELISA: 1:1,000 - 1:5,000, IHC: 1:50 - 1:250, IP: 1:50, WB: 1:1,000 - 1:5,000
Purity
Malate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart]. Cross reactivity against Malate Dehydrogenase from other sources may occur but has not been specifically determined.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Malate Dehydrogenase Peroxidase Conjugated has been tested by western blot and is useful in ELISA and immunohistochemistry. Optimal titers should be obtained by researchers.
Tested Applications
ELISA, IHC, IP, WB
NCBI Accession Number
P11708.4
Host or Source
Sheep
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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