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GOT1 Antibody

Aspartate Transaminase antibody

Product Specifications

Product Name Alternative

Sheep anti-Aspartate Transaminase Antibody, Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, ec 2.6.1.1 antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody, AST

UniProt

P00503

Reactivity

Porcine

Immunogen

Anti-Aspartate Transaminase antibody was produced by repeated immunizations with Aspartate Transaminase from pig heart.

Target

GOT1

Clonality

Polyclonal

Conjugation

Unconjugated

Dilution

ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000

Purity

Anti-Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Aspartate Transaminase (AST) [Pig Heart]. Cross reactivity against Aspartate Transaminase (AST) from other tissues and species may occur but have not been specifically determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Aspartate Transaminase is suitable for western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_999092.1

Host or Source

Sheep

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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