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ADH1, ADC1, YOL086C Antibody (Biotin)

Alcohol Dehydrogenase antibody (Biotin)

Product Specifications

Product Name Alternative

Rabbit anti-Alcohol Dehydrogenase Antibody biotin Conjugation, biotin conjugated rabbit ant-Alcohol Dehydrogenase Antibody, Alcohol dehydrogenase 1, Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, ADH, ADC

UniProt

P00330

Reactivity

Yeast

Immunogen

Alcohol Dehydrogenase [Yeast]

Target

ADH1, ADC1, YOL086C

Clonality

Polyclonal

Conjugation

Biotin

Concentration

1.0 mg/mL

Dilution

ELISA: 1:2,000 - 1:15,000, IP: 1:100, WB: 1:500 - 1:5,000

Purity

Anti-Alcohol Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from other sources may occur but have not been specifically determined.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Alcohol Dehydrogenase Biotin Conjugated Antibody has been tested in ELISA and Western blot. This product is assayed against 1.0 ug of Alcohol Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 100 μL

Tested Applications

ELISA, IP, WB

NCBI Accession Number

12643994

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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