Peroxidase (Horseradish) Antibody Biotin Conjugated
Peroxidase (Horseradish) antibody (Biotin)
Product Specifications
Product Name Alternative
Rabbit anti-Horseradish Peroxidase Antibody Biotin Conjugation, rabbit anti-HRP Antibody Biotin Conjugation, Biotin Conjugated rabbit anti-HRP Antibody, Peroxidase Biotin Conjugated antibody, Anti-Peroxidase BAC Antibody
Immunogen
Peroxidase [Horseradish]
Clonality
Polyclonal
Conjugation
Biotin
Concentration
1.0 mg/mL
Dilution
IHC: User Optimized, WB: User Optimized
Purity
Anti-Peroxidase (Horseradish) Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other sources is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Peroxidase Antibody Biotin Conjugated has been tested by dot blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Tested Applications
DOT
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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