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Gox Antibody (Biotin)

Glucose Oxidase antibody (Biotin)

Product Specifications

Product Name Alternative

Rabbit anti-Glucose Oxidase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

UniProt

P13006

Reactivity

Fungi

Immunogen

Glucose Oxidase [A.niger]

Target

Gox

Clonality

Polyclonal

Conjugation

Biotin

Field of Research

Metabolism Research

Concentration

1.0 mg/mL

Dilution

ELISA: User Optimized, WB: User Optimized

Purity

Anti-GLUCOSE OXIDASE Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [A.niger]. Cross reactivity against Glucose Oxidase from other sources may occur but have not been specifically determined.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-GLUCOSE OXIDASE Biotin Conjugated Antibody has been tested by ELISA and Western blot. This product is assayed against 1.0 μg of Glucose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:25,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 100 μL

Tested Applications

ELISA, WB

NCBI Accession Number

P13006.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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