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HXK2 Antibody (HRP)

Hexokinase antibody (Peroxidase)

Product Specifications

Product Name Alternative

Rabbit anti-Hexokinase antibody HRP conjugation, peroxidase conjugation rabbit anti-Hexokinase antibody, DKFZp686M1669 antibody, Hexokinase 2 antibody, Hexokinase 2 muscle antibody, Hexokinase type II antibody, HEX1, HKB

UniProt

P04806

Immunogen

Hexokinase [Yeast]

Target

HXK2

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Signal Transduction

Concentration

1.0 mg/mL

Dilution

ELISA: 1:1,000 - 1:2,500, WB: 1:500 - 1:1,000

Purity

Anti-HEXOKINASE (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast]. Cross reactivity against Hexokinase from other tissues and species is expected based on sequence homology.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-HEXOKINASE Peroxidase Conjugated Antibody has been tested by ELISA and western blot and can be used in applications such as Immunohistochemistry (IHC), and Immunofluorescence (IF) . Specific conditions should be optimized by researcher.

Tested Applications

ELISA, WB

NCBI Accession Number

CAA96973.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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