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CAT Antibody (HRP)

Catalase antibody (Peroxidase)

Product Specifications

Product Name Alternative

Rabbit anti-Catalase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Catalase Antibody, Cas 1 antibody, CAT antibody, Cs1 antibody, MGC138422 antibody, MGC138424 antibody

UniProt

P00432

Reactivity

Bovine

Immunogen

Catalase [Bovine Liver]

Target

CAT

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Metabolism Research

Concentration

1.0 mg/mL

Dilution

ELISA: 1:2,000 - 1:10,000, WB: 1:500- 1:2,500

Purity

Catalase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Catalase [Bovine Liver]. Cross reactivity against Catalase from other tissues and species may occur but have not been specifically determined.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Catalase Peroxidase Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Catalase [Bovine Liver] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, WB

NCBI Accession Number

AAI03067.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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