BMY1, AMYB Antibody (HRP)
Beta Amylase antibody (Peroxidase)
Product Specifications
Product Name Alternative
Rabbit anti-Beta Amylase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Beta Amylase Antibody, 1-4 alpha D glucan maltohydrolase antibody, 14 alpha D glucan maltohydrolase antibody, AMYB antibody, BMY1 antibody
UniProt
P10537
Reactivity
Plant
Immunogen
Beta Amylase [Sweet Potato]
Target
BMY1, AMYB
Clonality
Polyclonal
Conjugation
HRP
Field of Research
Stem Cell & Developmental Biology
Concentration
1 mg/mL
Dilution
WB: 1:250
Purity
Beta Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Beta Amylase [Sweet Potato]. Cross reactivity against Beta Amylase from other tissues and species may occur but have not been specifically determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Beta Amylase Peroxidase conjugated antibody has been tested by ELISA and dot blot This product is assayed against 1.0 ug of Beta Amylase [Sweet Potato] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:1,000 of the reconstitution concentration is suggested for this product.
Tested Applications
DOT, ELISA, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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