ADH1 Antibody (HRP)
Alcohol Dehydrogenase antibody (Peroxidase)
Product Specifications
Product Name Alternative
Rabbit anti-Alcohol Dehydrogenase Antibody Peroxidase Conjugation, HRP conjugated rabbit ant-Alcohol Dehydrogenase Antibody, Alcohol dehydrogenase 1, Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, ADH, ADC
UniProt
P00330
Reactivity
Yeast
Immunogen
Alcohol Dehydrogenase [Yeast]
Target
ADH1
Clonality
Polyclonal
Conjugation
HRP
Concentration
1.0 mg/mL
Dilution
ELISA: 1:4,000 - 1:20,000, IHC: 1:500 - 1:2,500, IP: 1:100, WB: 1:1,000 - 1:5,000
Purity
Alcohol Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from other sources is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Alcohol Dehydrogenase Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable in immunohistochemistry. Optimal titers are to be optimized by researchers.
Tested Applications
ELISA, IHC, IP, WB
NCBI Accession Number
12643994
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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