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SERPINB14 Antibody (FITC)

Ovalbumin antibody (FITC)

Product Specifications

Product Name Alternative

Rabbit anti-Ovalbumin Antibody FITC Conjugation, fluorescein conjugated rabbit anti-Ovalbumin antibody, Egg albumin antibody, Plakalbumin, Allergen Gal d II, Allergen=Gal d 2

UniProt

P01012

Reactivity

Gallus

Immunogen

Ovalbumin [Hen Egg White]

Target

SERPINB14

Clonality

Polyclonal

Conjugation

FITC

Concentration

1.0 mg/mL

Dilution

ELISA: 1:2,000 - 1:10,000, IF: 1:500 - 1:2,500, IP: 1:100, WB: 1:1,000 - 1:5,000

Purity

Ovalbumin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]. Cross reactivity against Ovalbumin from other tissues and species may occur but have not been specifically determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Ovalbumin fluorescein conjugated antibody has been tested by ELISA, dot blot, and western blot and is suitable for fluorescent western blot, immunofluorescence microscopy, and flow cytometry or FACS analysis as well as other antibody based fluorescent assays. Researchers should determine optimal titers for applications that are not stated below.

Tested Applications

DOT, ELISA, IF, IP, WB

NCBI Accession Number

P01012.2

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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