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B2M Antibody (Biotin)

Beta-2-Microglobulin antibody (Biotin)

Product Specifications

Product Name Alternative

Rabbit anti-Beta-2-Microglobulin Antibody biotin Conjugation, biotin conjugated Beta-2-Microglobulin Antibody, B2M antibody, Beta 2 microglobulin precursor antibody, Beta chain of mhc class 1 proteins antibody, Hdcma22p antibody

UniProt

P61769

Reactivity

Human

Immunogen

Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.

Target

B2M

Clonality

Polyclonal

Conjugation

Biotin

Field of Research

Immunology & Inflammation

Concentration

1.0 mg/mL

Dilution

ELISA: 1:2,000 - 1:10,000, IHC: 1:500 - 1:3,000, WB: 1:1,000 - 1:5,000

Purity

Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b2-Microglobulin [Human Urine]. Cross reactivity against b2-Microglobulin from other sources may occur but has not been specifically determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-beta-2-Microglobulin Biotin antibody has been tested by dot blot and is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Tested Applications

DOT, ELISA, IHC, WB

NCBI Accession Number

NP_004039.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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