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B2M Antibody (HRP)

Beta-2-Microglobulin antibody (Peroxidase)

Product Specifications

Product Name Alternative

Rabbit anti-Beta-2-Microglobulin Antibody Peroxidase Conjugation, HRP conjugated Beta-2-Microglobulin Antibody, B2M antibody, Beta 2 microglobulin precursor antibody, Beta chain of mhc class 1 proteins antibody, Hdcma22p antibody

UniProt

P61769

Reactivity

Human

Immunogen

Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.

Target

B2M

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Immunology & Inflammation

Concentration

5.0 mg/mL

Dilution

ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, IF: 1:200, WB: 1:500 - 1:2,000

Purity

Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum, as well as purified and partially purified b2-Microglobulin (Human Urine) . Cross reactivity against b2-Microglobulin from other sources is unknown.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-beta-2-Microglobulin Peroxidase antibody has been tested in ELISA and western blotting, and is suitable in IHC and IF. Researchers should determine optimal titers for applications that are not stated below. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 100 μL

Tested Applications

ELISA, IF, IHC, WB

NCBI Accession Number

NP_004039.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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