B2M Antibody (HRP)
Beta-2-Microglobulin antibody (Peroxidase)
Product Specifications
Product Name Alternative
Rabbit anti-Beta-2-Microglobulin Antibody Peroxidase Conjugation, HRP conjugated Beta-2-Microglobulin Antibody, B2M antibody, Beta 2 microglobulin precursor antibody, Beta chain of mhc class 1 proteins antibody, Hdcma22p antibody
UniProt
P61769
Reactivity
Human
Immunogen
Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.
Target
B2M
Clonality
Polyclonal
Conjugation
HRP
Field of Research
Immunology & Inflammation
Concentration
5.0 mg/mL
Dilution
ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, IF: 1:200, WB: 1:500 - 1:2,000
Purity
Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum, as well as purified and partially purified b2-Microglobulin (Human Urine) . Cross reactivity against b2-Microglobulin from other sources is unknown.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-beta-2-Microglobulin Peroxidase antibody has been tested in ELISA and western blotting, and is suitable in IHC and IF. Researchers should determine optimal titers for applications that are not stated below. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 100 μL
Tested Applications
ELISA, IF, IHC, WB
NCBI Accession Number
NP_004039.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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