TRBOTR Antibody (HRP)
Trypsin antibody (Peroxidase)
Product Specifications
Product Name Alternative
Rabbit anti-trypsin antibody HRP conjugation, peroxidase conjugated rabbit anti-trypsin antibody, Anionic trypsinogen antibody, Beta trypsin antibody, Brain trypsinogen antibody, Cationic Trypsinogen antibody, Digestive Zymogen antibody, Mesotrypsin antibody, Mesotrypsinogen antibody
UniProt
P00760
Reactivity
Bovine
Immunogen
Anti-Trypsin Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified.
Target
TRBOTR
Clonality
Polyclonal
Conjugation
HRP
Concentration
1 mg/mL
Dilution
WB: 1:100 - 1:500
Purity
Trypsin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsin [Bovine Pancreas]. Cross reactivity against Trypsin from other sources is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Trypsin Peroxidase Antibody has been tested by ELISA and dot blot and is suitable for western blotting. Researchers should determine optimal titers for applications that are not stated below.
Tested Applications
DOT, ELISA, WB
NCBI Accession Number
CAA38513.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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