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TRBOTR Antibody (HRP)

Trypsin antibody (Peroxidase)

Product Specifications

Product Name Alternative

Rabbit anti-trypsin antibody HRP conjugation, peroxidase conjugated rabbit anti-trypsin antibody, Anionic trypsinogen antibody, Beta trypsin antibody, Brain trypsinogen antibody, Cationic Trypsinogen antibody, Digestive Zymogen antibody, Mesotrypsin antibody, Mesotrypsinogen antibody

UniProt

P00760

Reactivity

Bovine

Immunogen

Anti-Trypsin Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified.

Target

TRBOTR

Clonality

Polyclonal

Conjugation

HRP

Concentration

1 mg/mL

Dilution

WB: 1:100 - 1:500

Purity

Trypsin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsin [Bovine Pancreas]. Cross reactivity against Trypsin from other sources is unknown.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Trypsin Peroxidase Antibody has been tested by ELISA and dot blot and is suitable for western blotting. Researchers should determine optimal titers for applications that are not stated below.

Tested Applications

DOT, ELISA, WB

NCBI Accession Number

CAA38513.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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