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HMW E.Coli Host Cell Proteins Antibody

HMW E.Coli Host Cell Proteins antibody

Product Specifications

Product Name Alternative

Rabbit anti-E.Coli Host Cell Protein antibody, rabbit anti-HCP antibody, rabbit Anti-Host Cell Protein, rabbit anti-HMW E.Coli HCP, rabbit anti-high molecular weight HCP

Reactivity

E. coli

Immunogen

Anti-HMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture high molecular weight (HMW) proteins from E.Coli (BL21) .

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Infectious Disease & Virology

Concentration

1.38 mg/ml

Dilution

ELISA: 1:1000-1:2000, WB: 1:500-1:1000

Purity

HMW Host Cell Protein A purified antibody is directed against high molecular weight E.coli Host Cell Proteins. The product was purified from monospecific antiserum by Protein A affinity chromatography.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-HMW Host Cell Proteins Antibody detects over 250 E.coli HMW proteins and has been tested in 2D-Page, western blotting, and ELISA.

Tested Applications

2D-PAGE, ELISA, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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