MST1R Antibody

Rabbit anti-RON antibody, anti-MSP receptor, CDw136, CD136, Macrophage-stimulating protein receptor, p185-Ron, PTK8, RON, Protein-tyrosine kinase 8, c-met-related tyrosine kinase

Anti-RON Antibody was produced in rabbits by repeated immunizations with a recombinant protein produced in Sf9 cells corresponding to the full length human RON protein.

Anti-RON antibody is directed against human RON. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-RON antibody was purified from rabbit serum by Protein A chromatography and cross adsorption of the GST tag. Cross reactivity with RON from other species has not been determined.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Anti-RON Antibody has been tested by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 152.3 kDa corresponding to full length protein by western blotting in the appropriate cell lysate or extract.