VlsE Antibody
VlsE antibody
Product Specifications
Product Name Alternative
Rabbit anti-VlsE Antibody, Outer surface protein VlsE, Borrelia burgdorferi VlsE, vlsE protein
UniProt
G5IXI6
Reactivity
Bacteria
Immunogen
MBP recombinant protein corresponding to Borrelia burgdorferi VlsE protein.
Target
VlsE
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:250, WB: 1:1000
Purity
This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi VlsE protein. A BLAST analysis was used to suggest reactivity with VlsE from B. burgdorferi sources based on 100% homology with the immunizing sequence. Cross-reactivity with VlsE from other sources has not been determined.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-VlsE antibody has been tested in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~36.3 kDa in size corresponding to VlsE by Western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
WP_010890281.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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