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VlsE Antibody

VlsE antibody

Product Specifications

Product Name Alternative

Rabbit anti-VlsE Antibody, Outer surface protein VlsE, Borrelia burgdorferi VlsE, vlsE protein

UniProt

G5IXI6

Reactivity

Bacteria

Immunogen

MBP recombinant protein corresponding to Borrelia burgdorferi VlsE protein.

Target

VlsE

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.0 mg/mL

Dilution

ELISA: 1:250, WB: 1:1000

Purity

This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi VlsE protein. A BLAST analysis was used to suggest reactivity with VlsE from B. burgdorferi sources based on 100% homology with the immunizing sequence. Cross-reactivity with VlsE from other sources has not been determined.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-VlsE antibody has been tested in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~36.3 kDa in size corresponding to VlsE by Western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, WB

NCBI Accession Number

WP_010890281.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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